Stage Micrometer Lab

⚗ Guided Lab

STEP 1 / 6 Calibration

1. OBJECTIVE LENS EYEPIECE ×10

Total magnification: ×100

2. CURRENT SLIDE ●

⌁ Stage micrometer

3. STAGE POSITION ↔

x +18 · y +12

4. FOCUS ADJUSTMENT ◌

COARSE

FINE

BLURRY

Show eyepiece graticule

WHY?

The eyepiece scale has no units yet

The graticule divisions are arbitrary: a cell spanning 20 divisions is not 20 µm. First compare the graticule with a stage micrometer of known size to discover the value of one division.

Medium-power calibration

Focus the scale, align it, then calculate the calibration.

Focus and align

Make both scales sharp and align their zero marks.

HOW TO DO THIS

LAB RECORD

Completed calibrations and measurements will appear here.

REFERENCE DATA

1 stage division: 10 µm

Eyepiece scale: arbitrary units

Specimens are calibrated teaching illustrations.

Use coarse focus to get close, then fine focus to make the markings sharp.

Lab reflection

Complete these two final reflections.

Why is calibration needed before measuring cells? How is a simulation like this useful, and what are its drawbacks compared with a real microscope?

Reflection saved on this device. Lab complete.